Ankom TDF Dietary Manual do Utilizador Página 1

Rev E 12/2/14 Operator’s Manual 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com

Operator’s Manual pg. 10 Rev E 12/2/14 Service Access Position If your instrument must be located in such a way as to restrict access to th

Operator’s Manual Rev E 12/2/14 pg. 11 Instrument Installation Site Requirements To install and operate the ANKOMTDF Dietary Fiber A

Operator’s Manual pg. 12 Rev E 12/2/14 1 – Pinch Valve Tubing Set (TDF71) 1 – Pump Tube Set (TDF68) 1 - Line Flush Tubing assy (TDF70) 1

Operator’s Manual Rev E 12/2/14 pg. 13 1 – Amylase/AMG Container assy (TDF57) (ONLY with AOAC 2009.01/2011.25 methods) 1 – Trizma Co

Operator’s Manual pg. 14 Rev E 12/2/14 7. Connect the Control Panel to the instrument. Remove the nuts from the right side of the instru

Operator’s Manual Rev E 12/2/14 pg. 15 10. Connect the Enzyme Containers to the corresponding ports. The Enzyme Containers conn

Operator’s Manual pg. 16 Rev E 12/2/14 Dietary Fiber Analysis Support Items The following support items needed to perform the fiber analysi

Operator’s Manual Rev E 12/2/14 pg. 17 IDF/SDF Analysis An IDF/SDF analysis measures the amount of IDF and SDF within a given sample

Operator’s Manual pg. 18 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 19 9.1.3 Press the ALL button to charge all of the lines. Each button will change color

This page intentionally left blank

Operator’s Manual pg. 20 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 21 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual pg. 22 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 23 12.4 Place at least 20 mm (0.75 inches) of the filter section of the IDF bag inside the top

Operator’s Manual pg. 24 Rev E 12/2/14 13.2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer. 13.3 Rep

Operator’s Manual Rev E 12/2/14 pg. 25 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

Operator’s Manual pg. 26 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 27 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 28 Rev E 12/2/14 21. Manually measure pH. One of the questions you are asked before starting the automated procedure

Operator’s Manual Rev E 12/2/14 pg. 29 22.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat seal

Table of Contents Introduction ...

Operator’s Manual pg. 30 Rev E 12/2/14 27. Determine the Protein content within the IDF and SDF residue. See the “Protein Determination” s

Operator’s Manual Rev E 12/2/14 pg. 31 TDF Analysis (AOAC 991.43) A TDF analysis directly measures the amount of TDF within a given

Operator’s Manual pg. 32 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 33 9.1.3 Press the ALL button to charge all of the lines. Each button will change co

Operator’s Manual pg. 34 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 35 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual pg. 36 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 37 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the to

Operator’s Manual pg. 38 Rev E 12/2/14 13.3 Repeat steps 13.1 – 13.2 for all IDF stations. 13.4 Re-install Clamp Bar A by setting it on t

Operator’s Manual Rev E 12/2/14 pg. 39 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

This page intentionally left blank

Operator’s Manual pg. 40 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 41 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 42 Rev E 12/2/14 21. Manually measure pH. One of the questions you are asked before starting the automated procedure

Operator’s Manual Rev E 12/2/14 pg. 43 23.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat seal

Operator’s Manual pg. 44 Rev E 12/2/14 This page intentionally left blank

Operator’s Manual Rev E 12/2/14 pg. 45 TDF Analysis (AOAC 985.29) A TDF analysis directly measures the amount of TDF within a given

Operator’s Manual pg. 46 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 47 9.1.3 Press the ALL button to charge all of the lines. Each button will change co

Operator’s Manual pg. 48 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 49 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual Rev E 12/2/14 pg. 5 Introduction ANKOM Technology designs, manufactures, and markets instruments and suppor

Operator’s Manual pg. 50 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 51 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the to

Operator’s Manual pg. 52 Rev E 12/2/14 13.3 Repeat steps 13.1 – 13.2 for all IDF stations. 13.4 Re-install Clamp Bar A by setting it on t

Operator’s Manual Rev E 12/2/14 pg. 53 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

Operator’s Manual pg. 54 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 55 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 56 Rev E 12/2/14 21. Adjust pH before the Protease digestion. Just before the Protease digestion (after the bags hav

Operator’s Manual Rev E 12/2/14 pg. 57 24. Rinse the SDF filter bags with acetone. After the instrument has completed a TDF

Operator’s Manual pg. 58 Rev E 12/2/14 28. Determine the Ash content within the TDF residue. See the “Ash Determination” section of this m

Operator’s Manual Rev E 12/2/14 pg. 59 Protein Determination AOAC method 991.43 suggests that the Kjeldahl method be used for dete

Operator’s Manual pg. 6 Rev E 12/2/14 Method Comparison (Manual vs. Automated) AOAC 991.43 Method – IDF/SDF assay without automation Below

Operator’s Manual pg. 60 Rev E 12/2/14 SDF / TDF – Kjeldahl Method To determine the protein content within the SDF or TDF residue, follow t

Operator’s Manual Rev E 12/2/14 pg. 61 Ash Determination ANKOM Technology recommends that you use ceramic crucibles with

Operator’s Manual pg. 62 Rev E 12/2/14 SDF / TDF During a TDF procedure there is no residue captured in the IDF Flow-Thru bag. Therefore

Operator’s Manual Rev E 12/2/14 pg. 63 Productivity Enhancement Start a new procedure while a previous one is in progress To enhance

Operator’s Manual pg. 64 Rev E 12/2/14 To start a new assay immediately after an IDF process completes, follow the steps below. 1. Prepare

Operator’s Manual Rev E 12/2/14 pg. 65 3. Install new SDF bags on the ANKOMTDF Dietary Fiber Analyzer and continue the run. When an

Operator’s Manual pg. 66 Rev E 12/2/14 Expert Mode – save some set-up time At the beginning of each new run, the instrument gives the

Operator’s Manual Rev E 12/2/14 pg. 67 Status Screen The screen below shows the primary sections of the instrument Status screen

Operator’s Manual pg. 68 Rev E 12/2/14 7. Bottom process – Name Box #7 always displays “SDF” to represent an SDF process within an ID

Operator’s Manual Rev E 12/2/14 pg. 69 The values shown on this screen will dynamically change as the temperatures change. When the

Operator’s Manual Rev E 12/2/14 pg. 7 Instrument Description The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurat

Operator’s Manual pg. 70 Rev E 12/2/14 This page intentionally left blank

Operator’s Manual Rev E 12/2/14 pg. 71 Fault Handling When the instrument detects a problem, it generates a fault. Some faults requi

Operator’s Manual pg. 72 Rev E 12/2/14 # Name Description Result Correction E4 In-line Heater Over Temp The device that heats the fluid bef

Operator’s Manual Rev E 12/2/14 pg. 73 Diagnostics Mode To help solve problems that may occur while running the ANKOMTDF D

Operator’s Manual pg. 74 Rev E 12/2/14 The value on the Mixer Duty Cycle screen is only used during Diagnostics and in an AOAC 2009.01/20

Operator’s Manual Rev E 12/2/14 pg. 75 Motor Test When you press the Motor Test button on the Diagnostics screen, the following scre

Operator’s Manual pg. 76 Rev E 12/2/14 Digestion Times When you press the Digestion Times button the following screen will be displayed. T

Operator’s Manual Rev E 12/2/14 pg. 77 When you press one of the Continue buttons, the Pump Tube Test screen is once again displayed

Operator’s Manual pg. 78 Rev E 12/2/14 Clear IDF/SDF Lines When you press the Clear IDF/SDF Lines button on the Diagnostics screen, the IDF

Operator’s Manual Rev E 12/2/14 pg. 79 Service Mode – IDF Count When you press the IDF Count button on the Service Mode screen, the

Operator’s Manual pg. 8 Rev E 12/2/14 External Components – Left Side View External Components – Right Side View Control Panel (TDF17)

Operator’s Manual pg. 80 Rev E 12/2/14 This page intentionally left blank

Operator’s Manual Rev E 12/2/14 pg. 81 Periodic Maintenance Inspect and clean the delivery nozzles at each IDF and SDF station On a

Operator’s Manual pg. 82 Rev E 12/2/14 This page intentionally left blank

Operator’s Manual Rev E 12/2/14 pg. 83 QC / Calibration Checks The ANKOMTDF Dietary Fiber Analyzer is an automated and highly integr

Operator’s Manual pg. 84 Rev E 12/2/14 3. Press the Volume Calibration button. The following screen will be displayed. 4. Label and wei

Operator’s Manual Rev E 12/2/14 pg. 85 6. Install extra plastic containers under all six IDF Delivery Nozzles to catch any fluid cu

Operator’s Manual pg. 86 Rev E 12/2/14 When the fluid delivery is complete, the following screen will displayed. 9. Remove the beakers w

Operator’s Manual Rev E 12/2/14 pg. 87 5. Press the Main Menu button. The following screen will be displayed.

Operator’s Manual pg. 88 Rev E 12/2/14 8. Press the Temperature Sensor Corrections button. The following screen will be displayed. 9. P

Operator’s Manual Rev E 12/2/14 pg. 89 Appendix A – Reagents (AOAC 991.43, AOAC 985.29) Use Distilled Water throughout. Solutions co

Operator’s Manual Rev E 12/2/14 pg. 9 Internal Components – Rear View N2 Check Valve (1 of 12) Main Electrical Cabinet ID

Operator’s Manual pg. 90 Rev E 12/2/14 Appendix B – Instrument Out-of-use Procedure The fluid lines can become obstructed when the

Operator’s Manual Rev E 12/2/14 pg. 91 2. Open the pump doors. 2.1 Unscrew the six acorn nuts on the back of the instrument

Operator’s Manual pg. 92 Rev E 12/2/14 Appendix C – De-fatting Procedure According to the AOAC 985.29E method (and referred to in the AOAC

Operator’s Manual Rev E 12/2/14 pg. 93 5. Soak the bags in Petroleum Ether. 5.1 Place all XT4 Filter Bags into a beaker or other

Operator’s Manual pg. 94 Rev E 12/2/14 Appendix D – Accessories (sold separately) Contact ANKOM Technology or an authorized dealer to order

This page intentionally left blank

Automation saves time and money! ANKOM Technology is an international company with products that include… A2000 Fiber Analyzer  Crude Fiber