
Hydrolysis Procedure
Total Fat Calculation
The hydrolysis procedure is done prior to fat extraction. When both hydrolysis and fat extraction are done, the Total
Fat contained within a food or feed sample can be calculated using the following formula:
100 x (W
2
– (W
3
+ (C
1
– C
2
)))
Original weight of sample
Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
Weight of dry extracted sample, filter bag, and DE
Blank filter bag dry weight after hydrolysis
Blank filter bag weight after extraction
Hydrolysis Support Items
The following support items are needed to perform the hydrolysis procedure:
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Bag Holder (used for adding sample to an empty filter bag)
Heat Sealer for sealing the filter bags
ANKOM #1915 (120V), #1920 (220V)
Multi-bag Holder (used to hold bags in the Hydrolysis Chamber)
Desiccant Pouch for drying
ANKOM #RD (120V), #RDI (220V)
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